By Blake W. Axelrod, Didier Wion (auth.), M.A. Hayat (eds.)
It is my wish that next volumes of the sequence will sign up for this quantity in helping within the extra whole figuring out of the motives, analysis, and cell-based remedy of significant human ailments and debilitating tissue/organ accidents. There exists a big, pressing call for by way of the general public and the clinical neighborhood to deal with to melanoma prognosis, therapy, therapy, and confidently prevention. Stem Cells are nature’s critical reward to multicellular organisms, together with people. The contents of the quantity are divided into six subheadings: Stem cellphone tradition, Bone Marrow Stem Cells, Mesenchymal Stem Cells, Reprogramming and Differentiation of Stem Cells, remedy, and Transplantation for the benefit of the readers.
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Additional info for Stem Cells and Cancer Stem Cells, Volume 8: Therapeutic Applications in Disease and Injury
The RGB method of sensing is of particular note for devising cell culture systems at a reasonable cost. Controlling pO2 at the pericellular level is possibly a greater challenge than measuring pO2. Oxygen diffusivity through water and PDMS (~5 × 10−5 cm2/s for both) is slow and thus it takes hours for changes in atmospheric oxygen levels to equilibrate at the bottom of a petri dish. Oxygen control systems for tissue culture with equilibration times on the order of a few minutes have been achieved in multiwell plates by bubbling the oxygen gas mixture directly in the well and by flowing oxygen through a thin PDMS insert in the well that avoids bubbles in the media (Oppegard et al.
2009). However, controlling pO2 in the gas phase of the incubator does not sufficiently regulate pO2 at the pericellular level. From a biological stand point only the pericellular pO2 experienced by cells is relevant. Unfortunately, controlling the pO2 in the gas phase of the incubator does not achieve this goal. Oxygen in the gas phase needs to diffuse into the medium. This can take, in the absence of stirring and depending of the depth of the medium, several hours. Moreover, measuring pO2 in the bulk of a culture medium only approximates pericellular pO2.
These sensors suffer from the same difficulties as optical glucose oxidase based sensors – consumption of glucose and dependence on pO2 at low oxygen levels. To address these issues the glucose sensor should be paired with an oxygen sensor and the sensor sizes must be optimized to minimize glucose consumption and hydroxide production without overly compromising sensitivity. Rodrigues et al. present a microfluidic chip with integrated electrochemical glucose and oxygen sensors located upstream and downstream of a cell culture chamber for measuring oxygen and glucose metabolism by the cells (Rodrigues et al.
Stem Cells and Cancer Stem Cells, Volume 8: Therapeutic Applications in Disease and Injury by Blake W. Axelrod, Didier Wion (auth.), M.A. Hayat (eds.)