New PDF release: Special Techniques for the Enzymologist

By Sidney P. Colowick, Nathan P. Kaplan

ISBN-10: 0121818047

ISBN-13: 9780121818043

The significantly acclaimed laboratory usual, equipment in Enzymology, is likely one of the so much hugely revered guides within the box of biochemistry. on account that 1955, each one quantity has been eagerly awaited, usually consulted, and praised through researchers and reviewers alike. The sequence includes a lot fabric nonetheless appropriate at the present time - really an important book for researchers in all fields of existence sciences.

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Example text

Whenever concentration determinations are to be made, either on purified components or in the analysis of mixtures, it is recommended that the double sector cell be employed. The solvent and solution patterns should be superimposed either from tracings of enlarged patterns (5X) made with a photographic enlarger or arithmetically if the areas are obtained by summing ordinates measured with a two-dimensional comparator. For this superposition of the patterns, use is made of the images corresponding to the reference holes in the counterbalance.

Yphantis 14 H. T. Epstein and M. A. Lauffer, Arch. Biochem. and Biophys. 35, 371 (1952). 1. For most enzymes, runs of a duration sufficient to produce a lowering of the average concentration in the upper solution to about 10% of the original will take only 2 or 3 hours at top speed, and the equation presented above is valid. If the molecules being studied are smaller, say about 2000 in molecular weight, then the plateau region will vanish in a 3-hour period and equation 9 is no longer applicable.

Thus the two separation cells functioning in quite different ways achieve, to more or less the desired extent, the same purpose. There is not as yet sufficient experience with the movable partition cell for a detailed comparison with the older cell, but it appears that the 62 TECHNIQUES FOR CHARACTERIZATION OF PROTEIN'S [2] newer cell will function better for proteins and low molecular weight materials, whereas the partition cell of Tiselius et al. does interfere with sedimentation and there is a partial disintegration of the sedimenting boundary as it approaches the partition from above.

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Special Techniques for the Enzymologist by Sidney P. Colowick, Nathan P. Kaplan


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