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By G. Klein (auth.), Dr. Michael Potter, Professor Dr. Fritz Melchers, Dr. Martin Weigert (eds.)

ISBN-10: 3642698603

ISBN-13: 9783642698606

ISBN-10: 364269862X

ISBN-13: 9783642698620

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Read Online or Download Oncogenes in B-Cell Neoplasia: Workshop at the National Cancer Institute, National Institute of Health, Bethesda, MD, USA, March 5–7, 1984 PDF

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Additional info for Oncogenes in B-Cell Neoplasia: Workshop at the National Cancer Institute, National Institute of Health, Bethesda, MD, USA, March 5–7, 1984

Sample text

3. Structure of CAT expression plasmids used to measure enhancer activity. pLPVl CATs contains the. , 1982) contains the SV40 enhancer element in similar position. Black squares denote location of the enhancer core sequences within the 63 bp and 72 bp repeat respectively. The 21 bp repeats (boxed) and TATA-box are part of the SV40 early promoter that is used in the expression of the gene coding for the bacterial enzyme Chloramphenicol-acetyltransferase. (CAT) • LPV SPECIFIC RECEPTOR IN TRANSFORMED B-LYMPHOCYTES ?

Nat'l. Acad. Sci. USA 76: 3665-3669. Kao, HT and Nevins, JR (1983) Transcriptional activation and subsequent control of the human heat shock gene during adenovirus infection. Mol. Cell. BioI. 3: 2058-2065. Kuff, EL and Fewell, JW (1980) Induction of neural-like cells in cultures of F9 teratocarcinoma treated with retinoic acid and dibutyryl cyclic adenosine monophosphate. Devel. BioI. 77: 103-115. Land, H, Parada, LF, and Weinberg, RA (19B3) Tumorigenic conversion of primary embryo fibroblasts requires at least two cooperating genes.

Expression of late LPV antigens (capsid proteins) 48 hours after transfection and infection was measured by indirect immunofluorescence using a rabbit serum raised against purified LPV particles. The T-lymphoma line Molt 4 and also mitogen stimulated peripheral blood lymphocytes are not productively infected by LPV particles but these cells express late LPV antigens when transfected with purified DNA (Table 2). ) that allows adsorption of LPV to the cell membrane,followed by penetration and uncoating of the viral DNA.

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Oncogenes in B-Cell Neoplasia: Workshop at the National Cancer Institute, National Institute of Health, Bethesda, MD, USA, March 5–7, 1984 by G. Klein (auth.), Dr. Michael Potter, Professor Dr. Fritz Melchers, Dr. Martin Weigert (eds.)


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